多肽單抗
服務編號:YKMS-002
服務標準:
1.1-3株雜交瘤細胞株
2.每株細胞提供2-5 mg抗體
3.抗體ELISA≥80,000
服務內容:
1.多肽合成(10mg,純度〉85%)
2.多肽偶聯(KLH用于免疫,BSA用于檢測)
3.免疫6只 Bal b/c小鼠,每只小鼠4-5次免疫,小鼠血清Elisa效價達到1:10000以上
4.取血清效價最高的小鼠的脾細胞與SP2/0進行細胞融合
5.3-4次亞克隆后得到穩定分泌特異性抗體的雜交瘤細胞株
6.雜交瘤細胞株擴增/凍存
7.小鼠腹水制備,Protein G純化得到抗體
8.抗體Elisa檢測效價,亞型鑒定
最終交付的材料:
1. 1-3株雜交瘤細胞株
2. 每株細胞提供2-5 mg Protein G純化的抗體
3. 1 mg多肽
4. Elisa數據報告,亞型鑒定報告,完整項目報告
Peptide → Monoclonal antibody
Service Including:
a.Peptide antigen design and preparation:
Peptide synthesis of up to 18 AA residues (20-30 mg) with a purity > 85%
b.Peptide conjugation:
Peptide conjugation to KLH and BSA
c.Immunization and serum titer test:
Pre-immune bleed
Six Balb/c mice immunization
Serum titer test
d.Fusion and Screening:
Fusion and Screening
Subcloning and cell expansion
e.Antibody production & purification:
Appropriate scale hybridoma cell culture
Purified by protein A affinity chromatography
Customer to provide:
Peptide Sequence
Delivery:
a.1-3 hybridoma cell lines;
b.2-5 mg protein A purified mAb per cell line.
c.mAb ELISA titer ≥80,000
Time:
20-26 weeks
電話:021-58955621
咨詢:youke_sh@sina.com
地址:蔡倫路720號1號樓339室