二抗在免疫學實驗中起著舉足輕重的作用,二抗的選擇也決定了整個實驗的成敗。實驗中在選擇二抗時必須注意以下幾點:(1) 一抗種屬來源,主要根據一抗種屬來源來決定確定二抗的類型,如一抗是小鼠來源,那二抗就買抗小鼠的即可(如羊抗小鼠、兔抗小鼠等均可)。
(2)標記物的選擇。一般來講,耦聯到二抗上的探針主要有酶(辣根過氧化酶HRP和堿性磷酸酶AP或其衍生物APAAP,PAP),熒光基團(FITC, RRX, TR, PE)和生物素。選用哪種探針的二抗主要取決于具體的實驗。對于western blot和ELISA,常用的二抗是酶標二抗,而細胞或組織標記實驗(細胞免疫化學,組織免疫化學,流式細胞術)中通常使用熒光基團標記的二抗,免疫組化中也可以使用辣根過氧化酶或堿性磷酸酶標記的二抗。如果想要更大程度的放大檢測信號,可以使用biotin/avidin檢測系統。
友科生物可以提供多種常規二抗,質優價廉,歡迎選購。
編號 | 識別的一抗種屬 | 名稱 | 應用 | 規格 |
YKGP201H | MOUSE IgG,HRP | Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP201F | MOUSE IgG,FITC | Anti-MOUSE IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP201B | MOUSE IgG,BIOTIN | Anti-MOUSE IgG (H&L) (GOAT) Antibody Biotin Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP202H | RABBIT IgG,HRP | Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP202F | RABBIT IgG,FITC | Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP202B | RABBIT IgG,BIOTIN | Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP203H | HUMAN IgG,HRP | Anti-HUMAN IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP203F | HUMAN IgG,FITC | Anti-HUMAN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP203B | HUMAN IgG,BIOTIN | Anti-HUMAN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKRP204H | GOAT IgG,HRP | Anti-GOAT IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKRP204F | GOAT IgG,FITC | Anti-GOAT IgG (H&L) (RABBIT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKRP204B | GOAT IgG,BIOTIN | Anti-GOAT IgG (H&L) (RABBIT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml |
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